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True solution phase measurements
KinExA technology measures the remaining free receptor molecule in the solution phase. Although a solid phase bound molecule is used for capture of the receptor, it is simply used as a trap for free receptor. Affinity for the solid phase bound molecule is not measured. Measuring binding events in the solution phase with micro beads for maximized surface area avoids mass transport limitations and mobility effects inherent to methods which measure binding to a solid phase. Mass transport limitations and mobility effects associated with binding to a solid phase are discussed by O’Shannessy and Winzor (Anal Biochem. 1996 May 1;236(2):275-83).
Measurement to unmodified molecules
KinExA technology measures binding affinity and rate constants for label-free, solution phase biomolecular interactions. While most methods for measuring binding events require at least one reactant be modified through immobilization or labeling, the KinExA method does not require modification of molecules under study. Therefore, concerns of how modification alters solution phase binding reactions are eliminated.
Unparalleled Sensitivity!
- The KinExA method allows a wider range of binding constants to be measured than other methods currently available. Binding affinities as tight as 10-13 M to date, on-rates as fast as 109 M-1s-1, and off-rates as slow as 10-7 can be measured accurately.
- KinExA sensitivity is not molecular size dependent.
Accuracy
The KinExA data analyses use exact solutions to classic binding equations (Affinity Constant Mathematics), not pseudo first-order approximations.
Easy to use
KinExA experiments are easy to set up and run automatically, without user intervention. No data manipulations, or arbitrary range selections, are required.
Cost
The KinExA 3000 system is regarded as the most affordable solution to accurate instrument based binding affinity and kinetic measurements. Contact a Sapidyne representative for a current price quote. The KinExA 3000 instrument is also inexpensive to maintain and use, costing only pennies per test.
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