Selected Publications

2005

Rathanaswami, P., Roalstad, S., Roskos, L., Su, Q.J., Lackie, S., Babcook, J. "Demonstration of an in vivo generated sub-picomolar affinity fully human monoclonal antibody to interleukin-8." BBRC 2005, 334, 1004-1013.

Xie, L., Jones, R.M., Glass, T.R., Navoa, R., Wang, Y., Grace, M.J. "Measurement of the functional affinity constant of a monoclonal antibody for cell surface receptors using kinetic exclusion fluorescence immunoassay." Journal of Immunological Methods 2005, 304, 1-14.

Razai, A., Garcia-Rodriguez, C., Lou, J., Geren, I.N., Forsyth, C.M., Robles, Y., Tsai, R., Smith, T.J., Smith L.A., Siegel, R.W., Feldhaus, M., Marks, J.D. "Molecular Evolution of Antibody Affinity for Sensitive Detection of Botulinum Neurotoxin Type A." J. Mol. Biol. 2005, 351, 158-169.

Sasaki, K., Glass, T. R., and Ohmura, N. "Validation of Accuracy of Enzyme-Linked Immunosorbent Assay in Hybridoma Screening and Proposal of an Improved Screening Method." Analytical Chemistry 2005, 77, 1933-1939.

2004
Darling, R.J, Brault, P.A. “Kinetic Exclusion Assay Technology: Characterization of Molecular Interactions.” ASSAY and Drug Development Technologies 2004, 2, 647-657.

Glass, T.R., Saiki, H., Blake, D.A., Blake II, R.C., Lackie, S.J., Ohmura, N. “Use of Excess Solid-Phase Capacity in Immunoassays: Advantages for Semicontinuous, Near-Real-Time Measurements and for Analysis of Matrix Effects.” Anal Chem 2004, 76, 767-772.

Glass, T.R., Saiki, H., Joh, T., Taemi, Y., Ohmura, N., Lackie, S.J. “Evaluation of a compact bench top immunoassay analyzer for automatic and near continuous monitoring of a sample for environmental contaminants.” Biosensors & Bioelectronics 2004, 20, 397-403.

2003
Blake II, R.C., J.B. Delehanty, M. Khosraviani, H. Yu, R.M. Jones, and D.A. Blake. “Allosteric Binding Properties of a Monoclonal Antibody and Its Fab Fragment”, Biochemistry 2003, 42, 497-508.

Carter, R. M., Blake, R. C., Nguyen, T. D., Bostanian, L. A. “Near Real-Time Biosensor-Based Detection of 2,4-Dinitrophenol”, Biosens Bioelectron 2003, 18, 69-72.

Delehanty, J. B., Jones, R. M., Bishop, T. C., Blake, D. A. “Identification of Important residues in Metal-Chelate Recognition by Monoclonal Antibodies”, Biochemistry 2003, 42, 14173-83.

Ohmura, N., Y. Tsukidate, H. Shinozaki, S.J. Lackie, and H. Saiki. “Combinational Use of Antibody Affinities in an Immunoassay for Extension of Dynamic Range and Detection of Multiple Analytes”, Analytical Chemistry 2003, 75, 104-110.

2002
Chin, T.E., R.B. Wong, J.L. Pont, and A.E. Karu. “Haptens and Monoclonal Antibodies for Immunoassay of Imidazolinone Herbicides”, J Agricultural and Food Chemistry 2002, 50, 3380-3389.

Jones R.M., H. Yu, J.B. Delehanty, and D.A. Blake. "Monoclonal Antibodies That Recognize Minimal Differences in the Three-Dimensional Structure of Metal-Chelate Complexes", Bioconjugate Chemistry 2002, 13, 408-415.

Nowakowski, A., Wang, C., Powers, D. B., Amersdorfer, P., Smith, T. J., Montgomery, V. A., Sheridan, R., Blake, R., Smith, L. A., and Marks, J. D. “Potent Neutralization of Botulinum Neurotoxin by Recombinant Oligoclonal Antibody” Proc. Natl. Acad. Sci. USA 2002, 99, 11346-50.

2001
Blake D.A., A.R. Pavlov, H. Yu, M. Khosraviani, H.E. Ensley, and R.C. Blake II. "Antibodies and Antibody-Based Assays for Hexavalent Uranium", Anal. Chim. Acta 2001, 444, 3-11.

Blake D.A., H. Yu, and R.C. Blake II. "Development of Rapid, Portable Immunoassays for Heavy Metals in Acid Mine Drainage", Biohydrometallurgy and the Environment- IBS 2001 (A. Ballerter and R. Amils, eds.) Elsevier, Amsterdam, 2001 Volume B, pp. 533-540.

Blake D.A., R.M. Jones, R.C. Blake II, A.R. Pavlov, I.A. Darwish, and H. Yu. "Antibody-Based Sensors for Heavy Metal Ions", Biosens. Bioelectron 2001, 16, 799-809.

Chiu, Y-W., Q.X. Li, and A.E. Karu. “Selective Binding of Polychlorinated Biphenyl Congerners by a Monoclonal Antibody: Analysis by Kinetic Exclusion Fluorescence Immunoassay”, Analytical Chemistry 2001, 73, 5477-5484.

Ohmura, N. S. Lackie, and H. Saiki. “An Immunoassay for Small Analytes with Theoretical Detection Limits”, Analytical Chemistry 2001, 73 (14), 3392-3399.

Ohmura, N. “Intercepting the Endocrine Disrupters”, Look Japan 2001, 46, 28-29.

Powers, D.B., P. Amersdorfer, M. Poul, U.B. Nielsen, M. Refaat Shalaby, G.P. Adams, L.M. Weiner, and J.D. Marks. “Expression of Single-Chain Fv-Fc Fusions in Pichia Pastoris”, Journal of Immunological Methods 2001, 251, 123-135.

2000
Carter, R.M. R.C. Blake II, H.P. Mayer, H.P. Echevarria, T.D. Nguyen, and L.A. Bostanian. “A Fluorescent Biosensor for Detection of Zearalenone”, Analytical Letters 2000, 33 (3), 405-412.

Eldefrawi, M.E., N.L. Azer, N. Nath, N.A. Anis, M.S. Bangalore, K.P. O’Connell, R.P. Schwartz, and J. Wright. “A Sensitive Solid-Phase Fluoroimmunoassay for Detection of Opiates in Urine”, Applied Biochemistry and Biotechnology 2000, 87, 25-35.

Hongo, J.S., S. Tsai, B. Moffat, K.A. Schroeder, C. Jung, A. Chuntharapai, P.A. Lampe, E.M. Johnson, F.J. De Sauvage, M. Armanini, H. Phillips, and B. Devaux. “Characterization of Novel Neutralizing Monoclonal Antibodies Specific to Human Neurturin”, Hybridoma 2000, 19 (4), 303-315.

Khosraviani, M., R.C Blake II, A.R. Pavlov, S.C. Lorbach, H. Yu, J.B. Delehanty, M.W. Brechbiel, and Blake, D.A. “Binding Properties of a Monoclonal Antibody Directed toward Lead-Chelate Complexes”, Bioconjugate Chemistry 2000, 11 (2), 267-277.

1999
Blake II, R.C., A.R. Pavlov, and D.A. Blake. “Automated Kinetic Exclusion Assays to Quantify Protein Binding Interactions in Homogeneous Solution”, Analytical Biochemistry 1999, 272, 123-134.

Chuntharapai, A., V. Gibbs, J. Lu, A. Ow, S. Marsters, A. Ashkenazi, A. De Vos, and K. JinKim. “Determination of Residues Involved in Ligand Binding and Signal Transmission in the Human IFN-" Receptor 2”, The Journal of Immunology 1999, 163 (2), 766-773.

Hanashima, T., N. Ohmura, H. Saiki, A. Ando, and Y. Nagata. In Analysis of Binding Events Between Lectins and Sugars Using Red Blood Cells Immobilized onto Solid Phase on Flow Fluorescence Spectrophotometer, Proceedings for 1999 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry (Nippon Nogeikagaku Kaiahi); Japan Society for Bioscience, Biotechnology, and Agrochemistry: Tokyo, 1999; 73, p 40. (Japanese Language)

O’Connell, K.P., J.J. Valdes, N.L. Azer, R.P. Schwartz, J. Wright, and M.E. Eldefrawi. “Assessment of an Automated Solid-Phase Competitive Fluoroimmunoassay for Benzoylecgonine in Untreated Urine”, Journal of Immunological Methods 1999, 225, 157-169.

Ohmura, N. N. Matsumoto, and H. Saiki. In Detection of Environmental Estrogens by Binding to Estrogen Receptor on Flow Fluorescence Spectrophotometer; Proceedings for 64th Annual Meeting of Chemical Engineering (Japan); Japan Society for Chemical Engineering: Tokyo, 1999; Vol. 1, p 476. (Japanese Language)

Ohmura, N., H. Saiki, and R.C. Blake II. In Direct Measurement of Binding Forces Between Antigen and Antibody Using Laser Trapping; Proceedings for 1999 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry (Nippon Nogeikagaku Kaiahi); Japan Society for Bioscience, Biotechnology and Agrochemistry: Tokyo, 1999, Vol. 73, p 379. (Japanese Language)

Ohmura, N., N. Matsumoto, K. Sasaki, T. Nagaoka, and H. Saiki. In Receptor Assay for Environmental Estrogens Using Flow Fluorescence Spectrophotometer; Proceedings for 1999 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry (Nippon Nogeikagaku Kaiahi); Japan Society for Bioscience, Biotechnology and Agrochemistry: Tokyo, 1999, Vol. 73, p 380. (Japanese Language)

Rogers, K.R., Y. Wang, A. Mulchandani, P. Mulchandani, and W. Chen. “Organophosphorous Hydrolase-Based Assay for Organophosphate Pesticides”, Biotechnology Progress, 1999, 15, 517-521.

1998
Blake, D.A., R.C. Blake II, M. Khosraviani, and A.R. Pavlov. “Immunoassays for Metal Ions”, Analytica Chimica Acta 1998, 376, 13-19.

Craig, L., P.C. Sanschagrin, A. Rozek, S. Lackie, L.A. Kuhn, and J.K. Scott “The Role of Structure in Antibody Cross-Reactivity Between Peptides and Folded Proteins”, Journal of Molecular Biology 1998, 281, 183-201.

Kaplan, B.E., L.J. Hefta, R.C. Blake II, K.M. Swiderek, and J.E. Shively. “Solid-phase Synthesis and Characterization of Carcinoembryonic Antigen (CEA) Domains”, Journal of Peptide Research 1998, 52, 249-260.

Rogers, K.R., Y. Wang, A. Mulchandani, P. Mulchandani, and W. Chen. “Development of a Versatile Organophosphorous Hydrolase-based Assay for Organophosphate Pesticides”, Proceedings of SPIE Conference on Environmental Monitoring and Remediation Technologies. Vo-Dinh, T.; Spellicy, R.L., Eds.; Boston, MA: The International Society for Optical Engineering, 1998, 3534, 420-428.

1997
Blake, D.A., M. Khosraviani, A.R. Pavlov, and R.C. Blake II. “Characterization of a Metal Specific Monoclonal Antibody”, D.S. Aga and E.M. Thurman, Eds.; ACS Symposium, Series 657, American Chemical Society, Washington, DC, 1997, pp 49-60.

Rogers, K.R., S.D. Kohl, L.A. Riddick, and T. Glass. “Detection of 2,4-Dichlorophenoxyacetic Acid Using a Fluorescence Immunoanalyzer”, The Analyst, 1997, 122, 1107-1111.

Strachan, N.J.C., P.G. John, and I.G. Millar. “Application of an Automated Particle-based Immunosensor for the Detection of Aflatoxin B1 in Foods”, Food and Agricultural Immunology 1997, 9, 177-183.

1996
Blake, D.A., P. Chakrabarti, M. Khosraviani, F.M. Hatcher, C.M. Westhoff, P. Goebel, D.E. Wylie, and R.C. Blake II. “Metal Binding Properties of a Monoclonal Antibody Directed Toward Metal-Chelate Complexes”, The Journal of Biological Chemistry 1996, 271 (44), 27677-27685.

1995
Glass, T. “Biospecific Binding and Kinetics-a Fundamental Advance in Measurement Technique.” Biomedical Products 1995, 20 (9), 122-123.

Strachan, N.J.C., D.I. Gray, I.G. Millar, J.C. Neidziela, and P.G. John. “Application of Fluorimetric Biosensors for the Rapid Detection of Food Pathogens by DNA- and Immuno- Assays”, Proceedings of Sensors and Their Applications VII, Dublin, Ireland, September 1995, 313-318.