If contamination is suspected, use this procedure to remove residual material from the Instrument Lines. This procedure is also recommended once a month to keep the Instrument clean.

Before cleaning the Instrument, the Pressure Transducer needs to be bypassed to avoid corrosion. See How to Guide 238 – Bypassing the Pressure Transducer (HG238) for instructions.

Note: The 4000 and other Instruments with a stainless steel Pressure Transducer do not require the Pressure Transducer to be bypassed.

Note: For 3X00 the various cleaning solutions should be placed on the Buffer Line, Inject Line, Sample Lines, and in the Particle Reservoir.

• Whenever a new solution is introduced, a Buffer Change procedure should be performed to put an air bubble between the solutions. Introducing a bubble to the Buffer Line reduces mixing of the new solution with the old.
• Click on the Buffer Change icon and follow the directions provided.
• Fill/Empty the Injection Syringe [2] times in order to prime the Syringe with each new solution.

Extreme Clean Procedure

1. Run [10] Rinses with a bleach solution*. Wipe Sample Lines with the bleach solution.
2. Overnight, run [25] Nightwashes with KinExA© Cleaning Solution (2T7010). Note: A full 250 mL bottle is needed.
3. Run [10] Rinses with Buffer of choice.

• Be sure to replace or sterilize all containers touching instrument fluids, including the Buffer Reservoir, Bead Vial, etc.

* It is important to keep the bleach solution below 0.5% NaOCl (sodium hypochlorite). If using household bleach, (~5% NaOCl) a 10 fold dilution is adequate. If using concentrated sodium hypochlorite solution (~10-15% NaOCl) a 20-30 fold dilution should be used.