KinExA® 4000
Introducing the KinExA 4000, the industry’s most precise and sensitive biosensor for determining the affinity and kinetics of biological interactions. Engineered for versatility, this cutting-edge platform enables accurate measurements of antibodies, native and engineered cells, small molecules, unpurified samples, and unmodified molecules in solution—delivering unmatched reliability for your research needs.
The KinExA 4000 eliminates the need for costly consumables such as chips, kits, or plates, optimizing your budget without compromising performance. With low sample volume requirements and experiments conducted in physiologically relevant, solution-phase conditions, it ensures both efficiency and scientific rigor.
Designed for ease of use, the KinExA 4000 integrates seamlessly with our intuitive KinExA Pro software, featuring single-click analysis to streamline workflows and accelerate insights. Elevate your research with a platform trusted by leading scientists worldwide.
Contact us today to schedule a personalized presentation or demonstration with one of our expert scientists and discover how the KinExA 4000 can transform your research.
ANTIBODIES
“To gain a more quantitative measurement of the antibody binding affinities to guide our epitope mapping experiments, we turned to KinExA analysis, a method that measures equilibrium binding affinities of unmodified molecules in the solution phase.”
— Wong X. et al., 2017. Fine epitope mapping of two antibodies neutralizing the Bordetella adenylate cyclase toxin. Biochemistry, 56(9), pp. 1324–1336
CELLS
“Having a robust methodology for characterizing antibody interaction with a membrane protein target is essential for providing guidance on dosing, potency, and thus expected efficacy… our study concluded that KinExA enables accurate measurement of the KD for strong protein–protein interactions (sub-nanomolar values) compared to FACS.”
— Vaish A. et al., 2020. Binding affinity determination of therapeutic antibodies to membrane targets: KinExA using cellular membranes for anti-CD20 antibody. Analytical Biochemistry, vol. 609
PROTEINS
“KinExA is particularly attractive to study protein–lipid interactions because native untagged molecules binding entirely in solution can be investigated.”
— Fleming J.K. et al., 2016. A novel approach for measuring sphingosine-1-phosphate and lysophosphatidic acid binding to carrier proteins using monoclonal antibodies and the kinetic exclusion assay. J. Lipid Research, 57(7), 1737–1747. doi:10.1194/jlr.D068866
SMALL MOLECULES
“The kinetics of binding and affinity of the 4 chimeric clones to human IL-21 were determined using a kinetic exclusion assay (KinExA)… Among the four lead clones, 19E3 had the highest affinity to recombinant human IL-21 of 243 fM.”
— Reena Varkey et al., 2019. Discovery and characterization of potent IL-21 neutralizing antibodies via a novel alternating antigen immunization and humanization strategy. PLoS ONE, 14(10): e0212236
DNA & APTAMERS
“Although numerous laboratory techniques have been developed to study the intricate interactions between proteins and DNA, each one of them has limited utility regarding applications. For better quantification of these important interactions, our work focused on using the Kinetics Exclusion Assay (KinExA) technology.”
— Leung E. et al., 2018. Quantifying protein–DNA interactions by kinetic exclusion assay. Biophysical Journal, 114(3): 442a
KinExA 4000 Features
KinExA 4000 |
Details |
|---|---|
| Applications | Affinity & Kinetics Determination, Concentration Analysis |
| Included with Purchase | KinExA 4000 Instrument, One Year Instrument Warranty, 10 Year LED Warranty, KinExA Pro Software with [2] Licenses, Installation & Custom Training, GxP Support |
| Automation | Accommodates up to [4] distinct solid phase preparations and, in conjunction with up to 270 samples, supports lengthy periods of autonomous operation. |
| Measure | Antibodies, Cells, Small Molecules, Proteins, DNA, Lipids, Viruses, Serum, & More |
| Analysis | With a single click using KinExA Pro Software, exact solutions to the binding equations are used to fit the theoretical binding curve to experimental data. 95% confidence intervals are included in the analysis, giving users an additional layer of confidence in results. |
| Measurement Range | fM to nM |
| Maintenance Costs | Features an updated plumbing package that reduces maintenance costs (compared to the KinExA 3200) and fits into the side panel of the instrument. Extended warranty and custom maintenance plans are available for an additional fee. |
| Consumables | Overall cost of consumables is low when compared to similar platforms. Basic lab items such as 1xPBS and sample tubes are used in each run and can be sourced from most lab vendors. Unique bead types can be purchased from Sapidyne or other vendors. Flow cells are replaced as needed but typically last a few hundred experiments before requiring replacement and can be purchased from Sapidyne. |
(2) Included
(3) When used with Autosampler
KinExA Specifications
Specifications
- Weight
37 kg - Power
36V DC
50-60Hz\
0.8A (max)
90W (max) - Power Recommendations
Uninterrupted Power Supply (UPS)
Minimum Specifications: 750 Watt - Interface
Ethernet - Operating Ambient Temperature
3°C – 40°C - Storage Temperature
3°C – 40°C - Environmental Conditions
No Direct Sunlight - Operating System
Windows 11