Ask the Inventor: Why do measurements need to be performed in the linear range? How will equilibrium & kinetic data be affected outside of linear range?
Publication Spotlight: Researchers at Regeneron describe how SPR capture surfaces can profoundly impact the binding kinetics that are measured for molecular interactions.
Ask the Inventor: In brief, what are the differences between KinExA & SPR?
Expanding KinExA Capabilities: Whole Cell Measurements, Direct Off Rate Measurement, Competitive Binding Analysis, & Positive Cooperativity Analysis
Ask the Inventor: The dielectric grease for the flow cell is messy and interferes with my camera images, do I really have to use it?
Tips & Tricks: Bead Coating
Publication Spotlight: Three for the Price of One - KinExA is used to measure the affinity for a combination of 3 antibodies against botulinum neurotoxin (BoNT)
Publication Spotlight: Measuring Sphingosine-1-Phosphate: Protein Interactions with the Kinetic Exclusion Assay
Tips & Tricks: Queue Cleaning Templates
Geek Corner: Exporting Charts
Ask the Inventor: Let's say I have a Kd controlled curve with an adequately resolved Kd. Why should I go to the trouble to perform a second experiment and use the n-curve analysis?
Ask the Inventor: The only way I can increase my
signal is to run very high volumes. The large
samples make painfully long experiment
run times, is there anything else I can do
to optimize my signal?
Publication Spotlight: Kinetic analysis of unpurified native antigens available in
very low quantities and concentrations.
Tips & Tricks: Pressure Transducer
Geek Corner: Exporting and Saving Experiment Options
Ask the Inventor:
Question 1: Sapidyne recommends performing my ligand serial dilution in receptor solution. To begin, I add concentrated ligand to the first sample then mix and do the serial dilution. Doesn't this mean it's possible my first sample (high concentration) may mostly bind up and I then have to wait for the dissociation (which is slower than association) for the subsequent samples to equilibrate?
Question 2: Sapidyne recommends a large volume serial dilution strategy in which I serial dilute convenient small volumes, again in receptor, with much higher ligand, then when the serial dilution is done dilute all the samples with receptor to the final concentration and volume. Again, I'm diluting solutions that could easily be mostly
bound so I have a longer wait for equilibrium, right?
